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MAB45765 PLCG2 Monoclonal Antibody

价格:    440.00元

  • Catalog:    MAB45765
  • Application:    WB,FCM,ICC, ELISA
  • Reactivity:    Human,Mouse,Rat

库存 800 件

MAB45765 PLCG2 Monoclonal Antibody

440.00元

Catalog : MAB45765
Application : WB,FCM,ICC, ELISA
Reactivity : Human,Mouse,Rat
Product Information


Product name

PLCG2 Monoclonal Antibody

Isotype

IgG2b

Form

Lyophilized or Liquid

Tested applications

WB,FCM,ICC, ELISA

Species reactivity

Human,Mouse,Rat

Host Species

Mouse

Purity

Affinity purification


Immunogen Information


Immunogen

Synthesized peptide of human PLCG2 (AA: phospho-Tyrosine 753 of human Phospholipase Cg2(cERDINSLpYDVSRMYV)).

Observed molecular weight 

148kDa

Gene ID (NCBI)

5336

Alternative Names

FCAS3; APLAID; PLC-IV; PLC-gamma-2 

 

Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:200 - 1:1000

FCM

1:200 - 1:400

ICC

1:200 - 1:1000

ELISA

1:10000



               图片关键词                              图片关键词    

Western blot analysis using PLCG2 mouse mAb against A431 cell lysate.


Flow cytometric analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).

    图片关键词           图片关键词                       

Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.


Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

                                      

Background


The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. 

 

Storage

Storage instructions

4℃; -20℃ for long term storage           Purified antibody in PBS with 0.05% sodium azide.