本试剂盒用于体外定量检测大鼠血清、血浆、组织、细胞上清及相关液体样本中肿瘤坏死因子α（TNF- α）的含量。
本试剂盒应用双抗体夹心法测定标本中大鼠肿瘤坏死因子α（TNF-α）水平。用纯化的大鼠肿 瘤坏死因子α（TNF-α）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入肿瘤坏死 因子α（TNF-α），再与HRP标记的肿瘤坏死因子α（TNF-α）抗体结合，形成抗体-抗原-酶标抗体 复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成 最终的黄色。颜色的深浅和样品中的肿瘤坏死因子α（TNF-α）呈正相关。用酶标仪在450nm波长下 测定吸光度（OD值），通过标准曲线计算样品中大鼠肿瘤坏死因子α（TNF-α）浓度。
反应类型 | 夹心法 | |||
规格 | 96T | |||
反应时间 | 2h | |||
反应性 | 大鼠 | |||
检测方法 | Colormetric | |||
检测范围 | 8pg/ml-640pg/ml | |||
灵敏度 | 最小可测 1.6 pg/mL | |||
样本体积 | 50μL | |||
样本类型 | 血清、血浆或其他相关生物液体 | |||
可检测样本中的大鼠肿瘤坏死因子α（TNF-α），且与其它相关蛋白无明显交叉反应
板内变异系数≤9%,板间变异系数≤11%
| 规格 | 96T | ||
说明书 | 1份 | ||
封板膜 | 2 片（96） | ||
96 孔酶标板 | 1×96 | ||
检测抗体(Detection Antibody) | 1.5ml×1 瓶 | ||
酶标试剂(Enzyme Reagent) | 6ml×1 瓶 | ||
标准品(Standards)：1280pg/ml | 0.6ml×1 瓶 | ||
测定稀释剂(Assay Diluent) | 2 ml×1 瓶 | ||
显色剂 A 液(Substrate Solution A) | 6ml×1瓶 | ||
显色剂 B 液(Substrate Solution B) | 6ml×1瓶 | ||
终止液(Stop Solution) | 6ml×1瓶 | ||
浓缩洗涤液(Wash Buffer ) | （20ml×30 倍）×1瓶 |
保存温度：2-8°C
需要而未提供的试剂和器材
1.吸光度值在450nm波长下检测的酶标仪
2.1-2ml的加样器
3.100 ml和1L的量筒
4.吸水纸
5.37°C 恒温箱
6.蒸馏水或去离子水
7.数据分析和绘图软件，图纸
8.标准和样品稀释的试管
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