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MU30030 MouseTumor necrosis factor α(TNF-α) ELISA Kit

价格:    2400.00元

  • Catalog:    MU30030
96T

库存 100 件

MU30030 MouseTumor necrosis factor α(TNF-α) ELISA Kit

2400.00元

Catalog : MU30030

用途         


用途

本试剂盒用于体外定量检测小鼠血清、血浆、组织、细胞上清及相关液体样本中肿瘤坏死因子α(TNF- α)的含量。


检测原理

本试剂盒应用双抗体夹心法测定标本中小鼠肿瘤坏死因子α(TNF-α)水平。用纯化的小鼠肿 瘤坏死因子α(TNF-α)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入肿瘤坏死 因子α(TNF-α),再与HRP标记的肿瘤坏死因子α(TNF-α)抗体结合,形成抗体-抗原-酶标抗体 复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成 最终的黄色。颜色的深浅和样品中的肿瘤坏死因子α(TNF-α)呈正相关。用酶标仪在450nm波长下 测定吸光度(OD值),通过标准曲线计算样品中小鼠肿瘤坏死因子α(TNF-α)浓度

反应类型

夹心法

规格

96T

反应时间

2h

反应

小鼠 

检测方法

Colormetric

检测范围

8pg/ml-640pg/ml

灵敏度

最小可测 1.6pg/mL

样本体积

50μL

样本类型

血清、血浆或其他相关生物液体


特异性

可检测样本中的小鼠中肿瘤坏死因子α(TNF-α),且与其它相关蛋白无明显交叉反应。


重复性

板内变异系数≤9%,板间变异系数≤11%


试剂盒组成及保存
规格
96T

说明书

1份

封板膜

2 片(96)

96 孔酶标板

1×96

检测抗体(Detection Antibody)

1.5ml×1 瓶

酶标试剂(Enzyme Reagent)

6ml×1 瓶

标准品(Standards):1280pg/ml

0.6ml×1 瓶

测定稀释剂(Assay Diluent)

2 ml×1瓶

显色剂 A 液(Substrate Solution A)

6ml×1瓶

显色剂 B 液(Substrate Solution B)

6ml×1瓶

终止液(Stop Solution)

6ml×1瓶

浓缩洗涤液(Wash Buffer )

(20ml×30倍)×1 瓶


保存温度:2-8°C 


实验室所需物品

需要而未提供的试剂和器材

1.吸光度值在450nm波长下检测的酶标仪;

2.1-2ml的加样器;

3.100 ml和1L的量筒;

4.吸水纸;

5.37°C 恒温箱;

6.蒸馏水或去离子水;

7.数据分析和绘图软件,图纸;

8.标准和样品稀释的试管‍。


操作步骤



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