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PAB40549 Symmetric DiMethyl-Histone H3-R8 pAb

价格:    580.00元

  • Catalog:    PAB40549
  • Application:    WB,IHC,IF,IP,ChIP,ChIPseq,ELISA
  • Reactivity:    Human,Mouse,Rat,Other (Wide Range)

库存 400 件

PAB40549 Symmetric DiMethyl-Histone H3-R8 pAb

580.00元

Catalog : PAB40549
Application : WB,IHC,IF,IP,ChIP,ChIPseq,ELISA
Reactivity : Human,Mouse,Rat,Other (Wide Range)
Product Information

 

Product name

Symmetric DiMethyl-Histone H3-R8 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF,IP,ChIP,ChIPseq,ELISA

Species reactivity

Human,Mouse,Rat,Other (Wide Range)

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3

Calculated molecular weight

15kDa

Observed molecular weight 

18kDa

Swiss Prot

Q16695

Gene ID (NCBI)

8290

Alternative Names

HIST3H3; H3.4; H3/g; H3FT; H3t; histone H3.1t


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:200

IP

1:50 - 1:200

CHIP

1:20 - 1:50

CHIPseq

1:20 - 1:50


         图片关键词               
图片关键词

Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R8 antibody.


Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R8 antibody.

 

      图片关键词

        Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R8 antibody.




Background


Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. 


Storage


Storage instructions

Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.