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PAB33583 Symmetric DiMethyl-Histone H3-R17 pAb

价格:    440.00元

  • Catalog:    PAB33583
  • Application:    WB,IHC,IF
  • Reactivity:    Human,Mouse,Rat,Other (Wide Range)

库存 800 件

PAB33583 Symmetric DiMethyl-Histone H3-R17 pAb

440.00元

Catalog : PAB33583
Application : WB,IHC,IF
Reactivity : Human,Mouse,Rat,Other (Wide Range)
Product Information

 

Product name

Symmetric DiMethyl-Histone H3-R17 pAb

Isotype

IgG

Form

Lyophilized or Liquid

Tested applications

WB,IHC,IF

Species reactivity

Human,Mouse,Rat,Other (Wide Range)

Host Species

Rabbit

Purity

Affinity purification


Immunogen Information


Immunogen

A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3

Calculated molecular weight

15kDa

Observed molecular weight 

17kDa

Swiss Prot

P68431

Gene ID (NCBI)

8356

Alternative Names

H3/j;H3FJ


Application Notes



The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB

1:500 - 1:2000

IHC

1:50 - 1:200

IF

1:50 - 1:200

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Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R17 antibody.


Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R17 antibody.

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Immunohistochemistry of paraffin-embedded human stomach using DiMethyl-Histone H3-R17 antibody.


Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 antibody.

Background


Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3


Storage


Storage instructions

Store at -20. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.